FRACTIONATION AND PROPERTIES OF GLUTAMIC-OXALACETIC TRANSAMINASE
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چکیده
منابع مشابه
Fractionation and properties of glutamic oxalacetic transaminase.
Because of the general nature of the transamination reaction (1, 2), a study of the kinetics and mechanism of reaction seemed of interest. Preliminary studies of this nature have already appeared (3-5). However, these studies were carried out with crude extracts or resolved enzyme systems. In order to conduct a critical kinetic study as well as a study of the mechanism of reaction, large amount...
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The determination of serum glutamic oxalacetic and glutamic pyruvic transaminase has been simplified by the use of automated analysis. The flow diagrams, concentration of reagents, and calculation of the color unit in terms of the Karmen unit are shown for 2,4-dinitrophenylhydrazine and 6-benzamido-4-methoxy-m -toluidine diazonium chloride as the reacting dyes. Advantages of both methods are co...
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THE ENZYME GLUTAMIC OXALACETIC ThANSAMINASE (GO-T), which serves as a catalyst for the reversible reaction of a-ketoglutarate and l-aspartate to produce oxalacetate and i-glutamate in biologic systems, has long been known to be a normal component of the serum proteins. The enzyme is widely distributed in animal and human tissues but its greatest activity has been found in homogenates of heart m...
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ARGE INCREASES in glutamic-oxalacetic transaminase (GOT) activity in blood serum have been reported for patients suffering from heart or liver tissue damage. The determination of this enzyme as described by Karmen (1) and modified by Steinberg, Baldwin, and Ostrow (2) couples the transaminase reaction with the malic dehydrogenase reaction wherein the disappearance of added reduced diphosphopyri...
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DIPHTHERITIC MYOCARDITIS BY E. CHESLER From the Johannesburg Fever Hospital, Johannesburg Received July 29, 1957 Serum glutamic oxalacetic transaminase (S.G.O.T.) is normally present in human serum, skeletal muscle, brain, liver, kidney, testis, and lung. Its highest concentration, however, is in cardiac muscle. Elevated S.G.O.T. levels occur with active dissolution of the tissues containing th...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1951
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)52427-9